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Int J Syst Evol Microbiol 59 (2009), 2734-2737; DOI  10.1099/ijs.0.010108-0
© 2009 International Union of Microbiological Societies

Corynebacterium doosanense sp. nov., isolated from activated sludge

Hee Joong Lee, Sung-Lim Cho, Min Young Jung, Thi Hai Van Nguyen, Yeon-Chang Jung, Hee Kuk Park, Van Phan Le and Wonyong Kim

Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea

Correspondence
Wonyong Kim
kimwy{at}cau.ac.kr

The taxonomic position of a Gram-positive, non-motile, non-spore-forming coryneform, isolated from activated sludge and designated strain CAU 212T, was investigated using a polyphasic approach. Cellular morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 212T had the characteristics of the genus Corynebacterium. Comparative 16S rRNA gene sequence analysis showed that the organism formed a hitherto-unknown subline within the genus Corynebacterium. Sequence divergence values of more than 4.3 % from recognized Corynebacterium species, together with phenotypic differences, showed that the bacterium represents a previously unrecognized member of the genus Corynebacterium, for which the name Corynebacterium doosanense sp. nov. is proposed. The type strain is CAU 212T (=KCTC 19568T=CCUG 57284T).

Abbreviations: CAMP, Christie–Atkins–Munch-Petersen

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CAU 212T is EU998655.






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