HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures All Versions of this Article: ijs.0.005827-0v1 59/8/1953    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Merhej, V. Articles by Roux, V. Search for Related Content PubMed PubMed Citation Articles by Merhej, V. Articles by Roux, V. Agricola Articles by Merhej, V. Articles by Roux, V.

Corynebacterium timonense sp. nov. and Corynebacterium massiliense sp. nov., isolated from human blood and human articular hip fluid

¹ Laboratoire de Bactériologie – Virologie, Hôpital de la Timone, URMITE CNRS-IRD UMR6236, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France
² Culture Collection, University of Göteborg, Department of Clinical Bacteriology, Guldhedsg.10, S-413 46 Göteborg, Sweden

Correspondence
Véronique Roux
vroux91{at}hotmail.com

Gram-positive, facultatively anaerobic, rod-shaped bacteria were isolated from the blood of a patient with endocarditis (strain 5401744^T) and from the hip joint fluid of a patient with an infected orthopaedic prosthesis (strain 5402485^T). These strains were characterized by using a polyphasic taxonomic approach. Based on cellular morphology and biochemical criteria the two isolates were tentatively assigned to the genus Corynebacterium, although they did not correspond to any recognized species. The predominant fatty acids were a mix of C_{18 : 2}6,9c and anteiso-C_{18 : 0} (32.1 % of the total), C_{16 : 0} (26.3 %) and C_{18 : 1}9c (22.5 %) for strain 5402485^T and C_{18 : 1}9c (36.4 %), C_{17 : 1}9c (27.1 %) and C_{16 : 0} (10.9 %) for strain 5401744^T. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain 5401744^T was closely related to the type strains of Corynebacterium auris, Corynebacterium capitovis, Corynebacterium lipophiloflavum and Corynebacterium mycetoides (97.0, 96.6, 96.5 and 96.3 % similarity, respectively) and strain 5402485^T was closely related to the type strains of Corynebacterium macginleyi, Corynebacterium accolens, Corynebacterium tuberculostearicum, Corynebacterium confusum, Corynebacterium mastitidis and Corynebacterium renale (95.6, 95.3, 95.3, 94.5, 94.0 and 93.5 %, respectively). On the basis of phenotypic data and phylogenetic inference, these isolates are considered to represent two novel species of the genus Corynebacterium, for which the names Corynebacterium timonense sp. nov. (type strain, 5401744^T=CSUR P20^T=CIP 109424^T=CCUG 53856^T) and Corynebacterium massiliense sp. nov. (type strain, 5402485^T=CSUR P19^T=CIP 109423^T=CCUG 53857^T) are proposed.

Electron micrographs of cells of strains 5401744^T and 5402485^T and neighbour-joining phylogenetic trees showing the position of strains 5401744^T and 5402485^T among representatives of the genus Corynebacterium inferred from 16S rRNA and rpoB gene sequence comparisons are available as supplementary material with the online version of this paper.