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Nocardia mikamii sp. nov., isolated from human pulmonary infections in the United States

¹ CDC, Atlanta, GA, USA;
² DSMZ, Germany

³ E-mail: jmb6{at}cdc.gov

Four nocardioform bacteria were isolated from clinical respiratory sources (W7467, W7811, W8061T and W9013) in the United States. Macroscopic examination showed scant aerial hyphae and beige-red substrate hyphae. They showed chemotaxonomic markers that were consistent with the classification of Nocardia: i.e., meso-diaminopimelic acid; arabinose and galactose as diagnostic sugars; the phospholipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; a menaquinone with a -cyclic isoprene side chain MK-8(H4cycl.); a fatty acid pattern composed of unbranched saturated and monounsaturated fatty acids with considerable amount of tuberculostearic acid; and mycolic acids comprising 52 to 62 carbon atoms with three principal mycolic acids which were mono- and polyunsaturated showing a chain length of C54, C56 and C58. 16S rRNA gene sequence data and phenotypic characters showed they were most closely related to Nocardia aobensis (DSM 44805T ). The G+C content was 68.3 mol %. Analysis of a 1,245-bp fragment of gyrB gene showed a clade separate from N. aobensis and was supported by the DNA relatedness of 67 % of W8061T to N. aobensis. These data indicated that the novel isolates represent a new species within the genus Nocardia, for which the name Nocardia mikamii sp. nov. is proposed, with W8061T (DSM 45174T = JCM 15508T) designated as the type strain.