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Published online ahead of print on 9 October 2009 as doi:ijs.0.018283-0
Int J Syst Evol Microbiol (2009); DOI 10.1099/ijs.0.018283-0
© 2009 International Union of Microbiological Societies
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Burkholderia acidipaludis sp. nov., aluminum-tolerant bacteria isolated from the Chinese water chestnut, Eleocharis dulcis, that grows in highly acidic swamps in Southeast Asia

Tomoko Aizawa1, Nguyen Bao Ve2, Pisoot Vijarnsorn3, Mutsuyasu Nakajima1 and Michio Sunairi1,4

1 Nihon University;
2 Can Tho University;
3 Royal Acid Sulfate Soil Improvement Experiment Station

4 E-mail: sunairi.michio{at}nihon-u.ac.jp

Two strains of aluminum-tolerant bacteria, SA33T and 7A078(BCC 36999), were isolated from the Chinese water chestnut (Eleocharis dulcis) that grows in highly acidic swamps (pH 2 - 4) in actual acid sulfate soil areas of Vietnam and Thailand, SA33T from Vietnam and 7A078 from Thailand. The strains were Gram-negative, aerobic, non-spore-forming, and rod-shaped bacteria, having a cellular width of 0.6 - 0.7 µm and a length of 1.3 - 1.7 µm. These strains showed good growth between pH 3.0 and 8.0 and between 17 and 37 °C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone and C16 : 0, C18 : 1{omega}7c, and C17 : 0 cyclo as their major fatty acids. Their fatty acid profiles are similar to those reported for other Burkholderia species. The DNA G+C content of these strains was 64 mol%. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Burkholderia. Although the calculated 16S rRNA gene sequence similarity values of SA33T to 7A078, B. kururiensis, B. sacchari, and B. tuberum were 100 %, 97.3 %, 97.1%, and 97.0 %, respectively, SA33T and 7A078 formed a group that was distinct in the phylogenetic trees, and the DNA-DNA relatedness values of SA33T to 7A078 and these other species were 90 %, 47 %, 46 %, and 45 %, respectively. The results of physiological and biochemical tests including whole-cell protein pattern analysis allowed phenotypic differentiation of these strains from the published Burkholderia species. Therefore, strains SA33T and 7A078 represent a new species, for which the name Burkholderia acidipaludis sp. nov. is proposed. The type strain is SA33T (= NBRC 101816T = VTCC-D6-6T).






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