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Haloactinobacterium album gen. nov., sp. nov. a novel halophilic actinobacterium isolated from a salt lake in China, with proposal of Ruaniaceae fam. nov.

¹ Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China;
² Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 8300;
³ Functional Metabolite Research Center KRIBB, 52 Eoeun-dong, Yuseong gu,

⁴ E-mail: wjli{at}ynu.edu.cn

A Gram-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated strain YIM 93306T, was isolated from a salt lake in Xinjiang province, north-west China, and was subjected to a polyphasic taxonomic study. The isolate grew in the presence of 2-16 % (w/v) NaCl and no growth occurred in the absence of NaCl. The peptidoglycan type was A4 with an L-Lys-L-Glu interpeptide bridge. The whole cell sugars were glucosamine, arabinose and mannose. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C15:0, anteiso-C15:0 and anteiso-C17:0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and one unknown phospholipid. The DNA G + C content was 68.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism fell within the radius of the suborder Micrococcineae, and that its closest phylogenetic neighbour was the type strain of Ruania albidiflava (96.2 % sequence similarity), the sole recognized species of the genus Ruania. The sequence similarities between strain YIM 93306T and members of other genera of the suborder Micrococcineae were lower than 95.2 %. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic differences, a novel genus and species, Haloactinobacterium album gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93306T (=DSM 21368T = KCTC 19413T =CCTCC AB 208069T). Based on phylogenetic characteristics and signature nucleotide patterns, the genus Ruania together with the newly suggested genus Haloactinobacterium are proposed to be classified as Ruaniaceae fam. nov.